DNA

Part:BBa_K1199049:Design

Designed by: Tian Wang   Group: iGEM13_UESTC_Life   (2013-09-17)

DhaA31-RBS-HheCW249P TCP->glycerol


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 744
    Illegal NgoMIV site found at 870
    Illegal AgeI site found at 1631
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

there is restriction site XhoI.


Source

DhaA31 is mutant of DhaA from Rhodococcus sp ,HheC-W249P is mutant of HheC from Agrobacterium radiobacter

References